Composite

Part:BBa_K4131013:Design

Designed by: Chun Ying Yu   Group: iGEM22_CCU_Taiwan   (2022-10-10)


The Cold-induced expressing cassette of pfa A and pfa D


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4351
    Illegal SpeI site found at 3866
    Illegal SpeI site found at 5324
    Illegal SpeI site found at 7433
    Illegal PstI site found at 2298
    Illegal PstI site found at 3240
    Illegal PstI site found at 5292
    Illegal PstI site found at 6783
    Illegal PstI site found at 7919
    Illegal PstI site found at 8731
    Illegal PstI site found at 9433
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4351
    Illegal NheI site found at 3823
    Illegal NheI site found at 5618
    Illegal NheI site found at 7307
    Illegal NheI site found at 9656
    Illegal NheI site found at 9979
    Illegal SpeI site found at 3866
    Illegal SpeI site found at 5324
    Illegal SpeI site found at 7433
    Illegal PstI site found at 2298
    Illegal PstI site found at 3240
    Illegal PstI site found at 5292
    Illegal PstI site found at 6783
    Illegal PstI site found at 7919
    Illegal PstI site found at 8731
    Illegal PstI site found at 9433
    Illegal NotI site found at 5681
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4351
    Illegal BglII site found at 4306
    Illegal BglII site found at 4612
    Illegal BglII site found at 5218
    Illegal BglII site found at 7068
    Illegal BglII site found at 7728
    Illegal BglII site found at 8594
    Illegal BamHI site found at 8006
    Illegal XhoI site found at 686
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4351
    Illegal SpeI site found at 3866
    Illegal SpeI site found at 5324
    Illegal SpeI site found at 7433
    Illegal PstI site found at 2298
    Illegal PstI site found at 3240
    Illegal PstI site found at 5292
    Illegal PstI site found at 6783
    Illegal PstI site found at 7919
    Illegal PstI site found at 8731
    Illegal PstI site found at 9433
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4351
    Illegal SpeI site found at 3866
    Illegal SpeI site found at 5324
    Illegal SpeI site found at 7433
    Illegal PstI site found at 2298
    Illegal PstI site found at 3240
    Illegal PstI site found at 5292
    Illegal PstI site found at 6783
    Illegal PstI site found at 7919
    Illegal PstI site found at 8731
    Illegal PstI site found at 9433
    Illegal AgeI site found at 4172
    Illegal AgeI site found at 4478
    Illegal AgeI site found at 5084
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2351


Design Notes

The pfa A and pfa D are the original version in Moritella marina


Source

The CspA promoter (BBa_K328001) and CspA 5' UTR (BBa_K2541308) are located at the 5' terminal of this composite part to regulate the pfa A and pfa D genes transcription. The t7 terminator (Bba_K3778018) is at the 3' terminal of this composite part.

The ribosome binding site (Bba_K3778013) is respectively added before pfa A and pfa D genes to mediated the individual protein translation.


References